What is a thermal cycler used for?

A thermal cycler, also known as a PCR machine, is used to amplify DNA and RNA segments via the polymerase chain reaction (PCR) by rapidly changing temperatures.

What maintenance is typically required for an Eppendorf MasterCycler?

Routine maintenance typically involves cleaning the block and exterior, and periodic calibration checks. Buyers should consult the manufacturer's manual for specific guidelines.

Can this unit be used for real-time PCR?

The standard Eppendorf MasterCycler is designed for endpoint PCR. Real-time PCR requires a specific real-time thermal cycler with fluorescence detection capabilities.

What consumables are needed for a thermal cycler?

Typical consumables include PCR tubes, strips, or plates, along with sealing films or caps, and PCR reagents such as enzymes, primers, and dNTPs.

Is this unit suitable for high-throughput applications?

While capable of processing multiple samples, for very high-throughput applications, systems with automated plate handling or multiple block configurations might be more efficient.

What should I check when buying a used thermal cycler?

Key checks include the condition of the thermal block, the functionality of the heated lid, the accuracy of temperature control, and the overall physical integrity of the unit. Always request a demonstration or service report if available.

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 1

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 2

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 3

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 4

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 5

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers, Foto 6

Used Eppendorf MasterCycler Thermal Cycler - PCR Machines & Thermal Cyclers

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Eppendorf MasterCycler Thermal Cycler

Name: Eppendorf MasterCycler Thermal Cycler
Brand: Eppendorf
SKU: 0726617
Availability: InStock

Preis: Preis auf Anfrage beim Verkäufer

Buy the Eppendorf MasterCycler Thermal Cycler direct-from-lab. Quality tested, excellent condition, and ready for immediate use.

Cambridgeshire, United Kingdom

5.773 km entfernt

Modellnummer

MasterCycler

Bestandsnummer

0726617

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Typische Informationen zu dieser Maschinenkategorie. Bitte bestätigen Sie die Einzelheiten immer beim Verkäufer.

DNA amplification for molecular biology research and diagnostics.
Gene expression analysis through reverse transcription PCR.
Genotyping for genetic studies and disease screening.
Cloning and mutagenesis procedures in biotechnology.
Pathogen detection in clinical and food safety laboratories.

Typical block format: Commonly features a 96-well block, though 48-well or 384-well formats are also found for this class of equipment.
Temperature range: Typically operates within a range of 4°C to 99°C.
Heating/cooling rate: For this class of equipment, heating rates commonly exceed 3°C/s and cooling rates can be over 2°C/s.
Power supply: Units of this type commonly require a standard laboratory power supply, typically 230V, 50/60Hz for the UK market.
Lid type: Typically features a heated lid to prevent condensation and ensure consistent reaction volumes.
Footprint: For this class of equipment, dimensions are typically compact, designed for benchtop use in a laboratory setting.

A thermal cycler, also known as a PCR machine, is used to amplify DNA and RNA segments via the polymerase chain reaction (PCR) by rapidly changing temperatures.

PCR machines and thermal cyclers are regarded as reliable workhorses in molecular biology laboratories. Buyers typically value precision temperature control, consistent cycling performance, and straightforward programming interfaces. When purchasing used equipment, inspectors should verify heating block accuracy, check thermal uniformity across wells, and confirm software compatibility with current laboratory systems. Common strengths include durability, ease of maintenance, and sustained performance across numerous applications in research, diagnostics, and quality control settings.

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