Becton Dickinson FACSCalibur Flow Cytometer

• Immunophenotyping for cell population analysis in clinical diagnostics.
• Cell cycle analysis and proliferation studies in research laboratories.
• Apoptosis detection and cell viability assessments.
• Intracellular cytokine staining for immune response evaluation.
• Stem cell research and rare event detection.

Typical throughput

Commonly processes samples at rates suitable for routine laboratory workloads, typically tens to hundreds of samples per day depending on assay complexity.

Number of colours detected

This model is known for 4-colour detection capabilities, allowing for multiparametric analysis.

Laser configuration

Units of this type typically feature multiple lasers, commonly blue (488 nm) and red (635 nm), for broad fluorochrome excitation.

Sample input

Typically accepts samples in standard flow cytometry tubes (e.g., 5 mL polystyrene round-bottom tubes) or microtiter plates with an optional autosampler.

Power supply

For this class of equipment, a standard laboratory power supply is required, typically 220-240V, 50Hz in the UK.

Footprint

Requires dedicated bench space; buyers should confirm exact dimensions with the seller for this specific unit.

• What software is typically used with a Becton Dickinson FACSCalibur?

  The Becton Dickinson FACSCalibur commonly operates with BD CellQuest Pro software for data acquisition and analysis. Other third-party analysis software may also be compatible.

• Is this unit suitable for sorting cells?

• What kind of maintenance does a used flow cytometer like this require?

• Can this system be upgraded for more colours?

• What consumables are needed for operation?

Particle counters and analysis systems are regarded as essential tools for quality assurance and contamination control across pharmaceutical, biotech and food processing sectors. When purchasing used units, buyers should verify calibration history and optical component condition, as these directly affect measurement accuracy. Industry users value their reliability and precision for meeting regulatory compliance standards. Key strengths include consistent performance across batch testing and minimal maintenance requirements when properly maintained.

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