Eppendorf 5420 Centrifuge

• Separation of biological samples in molecular biology and biochemistry laboratories.
• Pelleting of cells, bacteria, and yeast in microbiology.
• Purification of nucleic acids (DNA/RNA) and proteins.
• Preparation of samples for analytical techniques such as PCR and chromatography.

Typical capacity

This model typically accommodates microcentrifuge tubes (0.2 ml to 2.0 ml) and PCR strips, with rotor options for up to 24 tubes.

Maximum RCF

Units in this class commonly achieve RCFs exceeding 20,000 x g, suitable for demanding separation tasks.

Speed range

Typically offers speeds up to 14,000-15,000 rpm, depending on the rotor in use.

Temperature control

While the 5420 is a non-refrigerated model, it often features active fan cooling to minimise sample heating during operation.

Power supply

Commonly operates on standard laboratory power supplies, typically 230 V, 50/60 Hz for the UK market.

Footprint

As a benchtop unit, it is designed for a compact footprint, suitable for laboratory benches with limited space.

• What types of rotors are compatible with the Eppendorf 5420?

  The Eppendorf 5420 is typically compatible with a range of fixed-angle rotors designed for microcentrifuge tubes (0.2-2.0 ml) and PCR strips. Buyers should confirm specific rotor availability with the seller.

• Is this model suitable for temperature-sensitive samples?

• What maintenance is typically required for a used centrifuge like this?

• Can this centrifuge be used for clinical applications?

• What safety features are commonly found on this model?

Centrifuges are valued across laboratories and biotech facilities for reliable sample separation. Buyers typically prioritise rotor compatibility, temperature stability in refrigerated models, and ease of maintenance when purchasing used units. Industry sentiment reflects appreciation for durable construction and consistent performance. Key considerations include verifying motor condition, checking calibration history, and confirming availability of compatible rotors. Well-maintained examples offer strong value and longevity, though wear on seals and bearings warrants inspection. Speed range and capacity alignment with intended applications remains important for operational efficiency.

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