Roche LightCycler II Thermal Cycler

• Gene expression analysis in research and diagnostic laboratories.
• Pathogen detection and quantification in clinical and food safety settings.
• Genotyping and mutation analysis for genetic research.
• Drug discovery and development for pharmaceutical applications.

Typical reaction volume

Commonly supports reaction volumes in the microlitre range, typically 10-20 µL.

Throughput

For this class of equipment, throughput is generally suited for medium-volume sample processing.

Temperature range

Typically operates across a broad temperature range suitable for PCR, commonly 35°C to 95°C.

Heating/cooling rate

Units of this type are known for rapid heating and cooling rates to minimise cycle times.

Power supply

Commonly requires a standard laboratory power supply, typically 230V, 50Hz for UK operation.

Footprint

Generally designed for benchtop use, with a compact footprint for laboratory environments.

• What types of PCR can this unit perform?

  This unit is primarily designed for real-time quantitative PCR (qPCR), allowing for both qualitative and quantitative analysis of nucleic acids.

• Is specialised software required to operate this thermal cycler?

• What maintenance is typically required for a used unit?

• Can this unit be integrated with automated liquid handling systems?

• What consumables are compatible with the Roche LightCycler II?

PCR machines and thermal cyclers are regarded as reliable workhorses in molecular biology laboratories. Buyers typically value precision temperature control, consistent cycling performance, and straightforward programming interfaces. When purchasing used equipment, inspectors should verify heating block accuracy, check thermal uniformity across wells, and confirm software compatibility with current laboratory systems. Common strengths include durability, ease of maintenance, and sustained performance across numerous applications in research, diagnostics, and quality control settings.

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