Spectramax M2 Microplate reader

• ELISA assays for immunology and infectious disease research.
• DNA and RNA quantification in molecular biology workflows.
• Protein quantification using methods like Bradford or BCA assays.
• Enzyme kinetics studies to determine reaction rates and mechanisms.
• Cell-based assays for viability, proliferation, and cytotoxicity.

Plate Formats

Typically accommodates 6- to 384-well microplates, with options for 96- and 384-well being most common.

Detection Modes

For this model, absorbance and fluorescence detection are standard, with some units offering luminescence as an optional third mode.

Wavelength Range

Absorbance typically covers UV-Vis range (e.g., 200-1000 nm); fluorescence typically uses filters or monochromators for excitation and emission.

Temperature Control

Commonly includes temperature control up to 45°C or 60°C for temperature-sensitive assays.

Shaking Options

Typically offers linear, orbital, and double-orbital shaking modes for mixing reagents.

Software

Units of this type are typically controlled by proprietary software for data acquisition and analysis.

Power Supply

Commonly operates on standard laboratory power, typically 100-240V AC, 50/60 Hz.

Footprint

For this class of equipment, it is a compact benchtop unit, suitable for most laboratory environments.

• What types of assays can this microplate reader perform?

  This model is versatile, capable of performing a wide range of assays including ELISA, DNA/RNA/protein quantification, enzyme kinetics, and various cell-based assays, leveraging its absorbance and fluorescence detection capabilities.

• Is this unit suitable for high-throughput screening?

• What maintenance is typically required for a microplate reader?

• Can this reader be integrated with laboratory automation systems?

• What is the difference between the M2 and M2e models?

Microplate readers remain essential laboratory instruments valued for their reliability and consistency in high-throughput screening applications. Buyers typically prioritise optical performance, wavelength range compatibility, and software functionality when evaluating used models. Key considerations include detector condition, calibration currency, and spare parts availability. The equipment generally performs well in pharmaceutical development, diagnostics, and research settings. Reputation centres on durability and cost-effectiveness, particularly when sourced from well-maintained laboratory environments with complete documentation.

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