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Categoría del producto: PCR Machines & Thermal Cyclers
PCR Machines & Thermal Cyclers
The thermal cycler is the engine of PCR, driving the sample through repeated heating and cooling steps that double the target sequence with each cycle. This category brings together PCR machines and thermal cyclers of every kind, from standard endpoint models and gradient blocks to real time qPCR instruments that read amplification as it happens. Block capacity, ramp rate and, on real time units, the number of optical channels decide which format suits an assay. Whether you need a spare bench model or a real time platform for quantitative work, the listings here come from verified sellers within the molecular biology and genomics range, with configuration and condition set out plainly.
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Categoría del producto: PCR Machines & Thermal Cyclers
Techne TC 3000 Well Thermal Cycler
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Applied Biosystems - 2009 Applied Biosystems 2720 Thermal Cycler
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Qiagen Rotor-Gene Q PCR Parts Only
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MJ Research PTC-225 Thermal Cycler
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Roche LightCycler II Thermal Cycler
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Categoría del producto: PCR Machines & Thermal Cyclers
Eppendorf Mastercycler X50 Thermocycler
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Categoría del producto: PCR Machines & Thermal Cyclers
Bio-Rad Laboratories - Bio-Rad C1000 Touch Thermal Cycler
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Marcas de confianza
150 GBP
Precio desde
100 %
Vendedores verificados
24/7
Mercado global
Thermal Cycler Types
These instruments range from simple endpoint blocks to multiplex real time systems. The main types below cover routine and quantitative PCR alike.
Standard endpoint thermal cyclers
Endpoint models run the amplification and leave detection to a downstream step such as a gel. They are the dependable everyday choice for cloning, genotyping and library prep amplification.
Gradient thermal cyclers
Gradient blocks apply a range of annealing temperatures across the block in one run. This lets you optimise a new primer set quickly without a series of separate runs.
Real time qPCR instruments
Real time systems track amplification cycle by cycle using fluorescent chemistry for quantitative results. Optical channel count sets how many targets you can multiplex in a single well.
High throughput and fast block cyclers
Fast blocks and 384 well formats cut run time and raise capacity for busy labs. Ramp rate is the figure that separates a fast instrument from a standard one.
Brands on Machinery Masters
These instruments on the marketplace come from the major PCR instrument manufacturers and specialist makers. The strip below is built from the PCR Machines & Thermal Cyclers listings live on Machinery Masters, so the names move with available stock.
Últimos anuncios de PCR Machines & Thermal Cyclers
Eppendorf Mastercycler X50 Thermocycler
2750 GBPde segunda mano
MJ Research PTC-225 Thermal Cycler
595 GBPde segunda mano
Applied Biosystems Veriti 96-Well 9903 Thermal Cycler
1450 GBPde segunda mano
2009 Applied Biosystems 2720 Thermal Cycler
150 GBPde segunda mano
BD Max PCR Machine
15.000 GBPde segunda mano
Techne TC 3000 Well Thermal Cycler
200 GBPde segunda mano
Por qué elegir Machinery Masters
Solo vendedores verificados
Cada vendedor se verifica antes de que se publique un solo anuncio. Machinery Masters también revisa la exactitud de los anuncios, para que los compradores puedan invertir con confianza en maquinaria de alto valor.
Alcance global
Los anuncios proceden de vendedores de toda Europa, Norteamérica y otros lugares. Ese alcance amplía las opciones más allá de cualquier red regional de distribuidores y, a menudo, es la única forma de encontrar modelos descatalogados en buen estado.
Opciones de financiación flexibles
Los anuncios que cumplen los requisitos ofrecen financiación a través de proveedores asociados. Las condiciones se muestran directamente en las páginas de producto correspondientes, repartiendo el coste en lugar de agotar el capital circulante de una sola vez.
Contacto directo con el vendedor
No hay ningún intermediario entre el comprador y el vendedor. Pregunte por el historial de mantenimiento, organice inspecciones y negocie directamente con la persona que realmente conoce la máquina.
Applications and Uses
PCR is one of the most widely used techniques in the life sciences, so these instruments appear across research and testing. Here are their core uses.
Pathogen detection and diagnostics
Real time qPCR quantifies viral and bacterial nucleic acid for clinical and public health testing. Reliable optics and even block heating protect the sensitivity of these assays.
Genotyping and mutation analysis
Endpoint and real time instruments amplify targets for SNP and variant detection. Gradient blocks help tune assays for tricky templates.
Cloning and library construction
Standard blocks amplify inserts and fragments through cloning and sequencing library prep. A dependable machine is a bench staple in any molecular lab.
Gene expression studies
Real time qPCR measures transcript levels to compare expression across conditions. Consistent cycle to cycle performance underpins reliable quantification.
Benefits from Machinery Masters
The marketplace lists thermal cyclers next to the extraction and sequencing steps that bracket them.
01
Endpoint and real time formats side by side
The category covers everything from a basic block to a multiplex real time system. Comparing formats directly helps you buy the right level of instrument for the assay.
02
Refurbished thermal cyclers that free up budget
A serviced, used unit runs the same protocols for much less than a new one. Listings state block format, options and condition so the saving is easy to weigh.
03
Direct contact on optics and calibration
You can ask the seller about optical channel status and recent calibration before buying a real time instrument. That conversation matters more than the headline specification.
Frequently Asked Questions sobre PCR Machines & Thermal Cyclers
Ask for the run count and confirm the block still heats and cools evenly with no dead wells. On a real time unit, verify the optical channels calibrate cleanly and the excitation source is within its rated life. A recent verification run from the seller is the best evidence the instrument still performs.
Match the block to your typical run, whether that is 96 well plates, strip tubes or a 384 well format for high throughput. Consider ramp rate if run time matters, since fast blocks shave minutes off every cycle. A gradient block is worth having if you optimise new assays often.
If you only amplify and then check on a gel, an endpoint model is enough and costs less. Quantitative work, pathogen load and expression studies need a real time qPCR system with fluorescence detection. Decide based on whether you must measure amplification as it happens or only confirm it afterwards.
Confirm the block accepts your plate or tube format and that any real time chemistry matches the optical filters. Some fast protocols also need specific low profile plates. Checking consumable fit upfront avoids adapters and reagent changes later.
Financing is available on qualifying listings, which helps spread the cost of a real time cycler. Where it applies, the option is shown on the listing. You can also raise it with the seller when you enquire to see how it fits your budget.
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