Cytiva Axichrom 140/300 chromatography column

• Biopharmaceutical purification, including proteins, antibodies, and vaccines.
• Separation and purification of active pharmaceutical ingredients (APIs).
• Food and beverage processing for ingredient purification and analysis.
• Chemical and petrochemical industries for fine chemical separation.
• Research and development in life sciences and biotechnology.

Column diameter

This specific model, the 140/300, indicates a nominal diameter of 140 mm.

Column length

The 140/300 model typically refers to a bed length of 300 mm, though this can be adjustable or fixed depending on the specific configuration.

Operating pressure

AxiChrom columns are designed for low-pressure chromatography, typically operating up to 20 bar (290 psi) for this class of equipment.

Materials of construction

Commonly, wetted parts are made from biocompatible materials such as 316L stainless steel, PEEK, and specific elastomers, suitable for pharmaceutical applications.

Temperature range

Typically designed to operate within a range of 4°C to 40°C, suitable for bioprocess applications.

Flow rate

Achievable flow rates are dependent on column diameter, packed bed characteristics, and operating pressure, but are generally suitable for process-scale purification.

• What is the typical bed volume for a Cytiva AxiChrom 140/300 column?

  For a 140 mm diameter and 300 mm bed length, the typical bed volume would be approximately 4.6 litres. Buyers should confirm the exact packed bed volume with the seller.

• Is this column suitable for cGMP environments?

• What type of chromatography resins can be used with this column?

• What maintenance is typically required for a used chromatography column?

• Are spare parts readily available for Cytiva AxiChrom columns?

Chromatography columns and accessories hold strong reputations within analytical laboratories for reliability and consistent performance. Buyers typically value used equipment that maintains original specifications and comes with documentation of maintenance history. Industry sentiment favours purchasing from reputable sources where column condition, packing integrity, and compatibility with existing systems are verified. Common strengths include durability of quality columns across multiple applications and cost savings versus new purchases. When buying used, inspecting for damage, confirming proper storage conditions, and verifying suitability for intended analytical methods remain critical considerations for laboratory operations.

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