Isoelectric Focusing Systems

Confirm the high voltage supply reaches and holds its rated output, since stable voltage is what gives sharp bands. Check the tray electrodes and any cooling plate are intact and free of salt corrosion. Ask for a recent run record to see the unit still resolves as expected.

Immobilised pH gradient strips give reproducible, defined ranges and are the usual choice for 2D proteomics. Narrow range strips resolve close variants, while broad range strips give a whole proteome overview. Match the strip range to the isoelectric points you need to separate.

Heat generated at high voltage can distort the gradient and blur bands, so many platforms cool the gel actively. If your protocol runs at high volt hours, a temperature controlled model is worth the extra cost. For shorter, lower voltage runs, passive cooling may be enough.

Trays use format specific strip holders and electrode wicks that must match the unit. Check these are included or still sold before you buy. The seller can confirm what ships with it and which consumables remain in production.

Every listing gives you a direct route to the seller, so you can ask about run history and included accessories straight away. There is no middleman between you and the owner. That direct contact is the quickest way to confirm a specialist instrument fits your protocol.