PCR Machines & Thermal Cyclers

Ask for the run count and confirm the block still heats and cools evenly with no dead wells. On a real time unit, verify the optical channels calibrate cleanly and the excitation source is within its rated life. A recent verification run from the seller is the best evidence the instrument still performs.

Match the block to your typical run, whether that is 96 well plates, strip tubes or a 384 well format for high throughput. Consider ramp rate if run time matters, since fast blocks shave minutes off every cycle. A gradient block is worth having if you optimise new assays often.

If you only amplify and then check on a gel, an endpoint model is enough and costs less. Quantitative work, pathogen load and expression studies need a real time qPCR system with fluorescence detection. Decide based on whether you must measure amplification as it happens or only confirm it afterwards.

Confirm the block accepts your plate or tube format and that any real time chemistry matches the optical filters. Some fast protocols also need specific low profile plates. Checking consumable fit upfront avoids adapters and reagent changes later.

Financing is available on qualifying listings, which helps spread the cost of a real time cycler. Where it applies, the option is shown on the listing. You can also raise it with the seller when you enquire to see how it fits your budget.