What types of samples can this centrifuge process?

This centrifuge is designed for micro-volume samples in molecular biology, such as DNA/RNA, proteins, and cell pellets, typically in 0.2 mL to 2.0 mL tubes.

Is this unit suitable for temperature-sensitive samples?

As a non-refrigerated model, it is best suited for samples that do not require active cooling during centrifugation. For temperature-sensitive applications, a refrigerated centrifuge would be more appropriate.

What maintenance is typically required for a centrifuge of this type?

Routine cleaning of the rotor and chamber, inspection of the rotor for wear, and ensuring the lid seal is intact are common maintenance tasks. Calibration checks may be recommended periodically.

Can this centrifuge accommodate different tube sizes?

Units of this class commonly come with a standard rotor for 1.5/2.0 mL tubes. Optional adapters or alternative rotors are typically available to accommodate smaller tubes (e.g., 0.2 mL, 0.5 mL) or PCR strips.

What safety features are typically included?

Common safety features include aerosol-tight rotors for containment of hazardous samples, lid interlocks to prevent operation when open, and automatic lid opening at the end of a run to prevent sample warming.

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 1

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 2

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 3

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 4

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 5

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 6

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 7

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 8

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical), photo 9

Used 5420 Centrifuge - Centrifuges (Benchtop, Refrigerated, Plate, Clinical)

used Equipment

Available

Verified seller

Eppendorf - 5420 Centrifuge

Name: 5420 Centrifuge
Brand: Eppendorf
SKU: 58017.SR12C
Availability: InStock

Price: Contact seller for price

This listing is for the Eppendorf Centrifuge 5420

The unit is in full working condition and is ready for immediate use

The Eppendorf 5420 is a non-refrigerated microcentrifuge designed for routine molecular biology workflows—especially spin‑column-based DNA/RNA purification, gel extraction, and PCR prep. It offers outstanding performance while saving precious bench space.

Key Features & Design Highlights

Compact Footprint: Measures just 24 × 35 × 22 cm (W × D × H), or approximately 9.4 × 13.8 × 8.7 in., making it ideal for crowded lab benches.

High-Speed Performance: Reaches up to 15,060 rpm, generating a maximum relative centrifugal force (RCF) of 21,300 × g.

Large Capacity with Flexibility: Comes standard with an aerosol‑tight rotor accommodating 24 × 1.5/2.0 mL tubes. Optional adapters support 0.2, 0.4, 0.5 mL tubes, 0.6 mL BD Microtainers, as well as a rotor for 32 × 0.2 mL PCR tubes or four 8‑strip PCR sets.

Ergonomic Controls:

Soft‑touch lid closure—one-finger operation.

Automatic lid opening at the end of runs to minimize sample warming and ease access.

One‑push short‑spin key—perfect for quick spins without holding the button.

Easy‑clean keypad and large, bright LCD display.

Low Noise & Safety:

Quiet operation at < 56 dB(A).

Aerosol‑tight rotor ensures safe handling of hazardous or sensitive samples.

Run Timer: Programmable from 10 seconds up to 9 hours, 59 minutes, and includes a continuous run mode.

Typical Applications

Spin‑column based DNA/RNA purification

Gel extraction and cleanup workflows

Preparation for PCR reactions

Handling of pathogenic or hazardous samples safely due to aerosol containment

Cambridgeshire, United Kingdom

5,791 km away

Model Number

5420

Stock Number

58017.SR12C

Machinery Masters View

Typical context for this category of equipment. Always confirm specifics with the seller.

Applications & Use Cases

Spin-column based DNA/RNA purification.
Gel extraction procedures.
PCR preparation workflows.
General molecular biology sample separation and processing.
Micro-volume sample pelleting and clarification.

Typical Specifications

Typical capacity: Commonly accommodates 24 x 1.5/2.0 mL tubes, with adapters for smaller volumes (e.g., 0.2, 0.4, 0.5 mL) or PCR strips.
Maximum speed: Units of this type typically reach up to 15,000 rpm.
Maximum RCF: For this class of equipment, maximum RCF is commonly around 21,000 x g.
Temperature control: This model is typically a non-refrigerated benchtop centrifuge.
Footprint: Compact designs are typical, often around 24 cm (W) x 35 cm (D) x 22 cm (H).
Noise level: Units of this specification commonly operate below 56 dB(A).
Power supply: Typically operates on standard laboratory mains power, commonly 230V, 50/60Hz for the UK market.

Frequently Asked Questions

This centrifuge is designed for micro-volume samples in molecular biology, such as DNA/RNA, proteins, and cell pellets, typically in 0.2 mL to 2.0 mL tubes.

Machinery Masters Intelligence. General guidance for this category of equipment only, not a substitute for the seller's exact specifications. Always confirm details with the seller.