What is Pyrosequencing?

Pyrosequencing is a real-time, sequence-based detection technology that measures the release of pyrophosphate during DNA synthesis. It is particularly effective for quantitative analysis of sequence variations and methylation.

What kind of maintenance does a PyroMark Q24 typically require?

Routine maintenance for this type of sequencer usually involves regular cleaning of fluidic paths, calibration checks, and replacement of consumables. Specifics should be detailed in the manufacturer's manual.

Can this unit be used for de novo sequencing?

Yes, the PyroMark Q24 is commonly used for de novo sequencing applications, particularly for microbial typing and characterisation where shorter read lengths are sufficient.

What are the typical consumables needed for a PyroMark Q24?

Consumables for Pyrosequencing typically include specific reagents (enzymes, substrates, nucleotides), PyroMark Q24 plates, and tips. Buyers should verify current availability and compatibility.

Is training usually required to operate this equipment?

Yes, due to the specialised nature of molecular biology equipment, operator training is typically recommended to ensure correct usage, data quality, and instrument longevity.

used Equipment

Available

Verified seller

Qiagen - PyroMark Q24 Sequencer

Name: PyroMark Q24 Sequencer
Brand: Qiagen
SKU: 49353664.1644
Price: 1700 GBP
Availability: InStock

Price: £ 1,700

This listing is for a Qiagen PyroMark Q24 Advanced Sequencer For advanced methylation and mutation quantification in long sequence runs using Pyrosequencing Advanced technology, software, and chemistry for long sequence runs Quantitative methylation analysis at consecutive CpG or CpN sites Improved quantification of sequence variations at any sequence position Easy and improved base calling functionality Assay versatility on the same instrument and in the same run PyroMark Q24 Advanced has improved Pyrosequencing technology to provide even better real-time sequence-based detection and quantification than before. PyroMark Q24 Advanced features advanced technology, software, and chemistry, and is highly suited for analyzing any kind of sequence variation, particularly DNA methylation at CpG or CpN sites, complex mutations, or for de novo sequencing applications such as microbial typing. Can be refurbished on request, refurbishment not included in price.

Cambridgeshire, United Kingdom

5,791 km away

Model Number

PyroMark Q24

Stock Number

49353664.1644

Machinery Masters View

Typical context for this category of equipment. Always confirm specifics with the seller.

Applications & Use Cases

Quantitative DNA methylation analysis at CpG or CpN sites.
Detection and quantification of single nucleotide polymorphisms (SNPs) and other sequence variations.
Microbial typing and characterisation.
De novo sequencing for short to medium read lengths.
Genetic analysis in research and clinical settings.

Typical Specifications

Typical throughput: Processes 24 samples simultaneously, offering efficient batch analysis for this class of sequencer.
Power supply: Commonly operates on standard laboratory power, typically 100-240 V AC, 50/60 Hz.
Footprint: Units of this type are designed for benchtop use, requiring a moderate amount of laboratory space.
Technology: Utilises Pyrosequencing technology for real-time, sequence-based detection and quantification.
Sample type: Typically processes purified DNA samples, prepared according to manufacturer protocols.
Software: Units commonly include dedicated software for instrument control, data acquisition, and analysis.

Frequently Asked Questions

Pyrosequencing is a real-time, sequence-based detection technology that measures the release of pyrophosphate during DNA synthesis. It is particularly effective for quantitative analysis of sequence variations and methylation.

Machinery Masters Intelligence. General guidance for this category of equipment only, not a substitute for the seller's exact specifications. Always confirm details with the seller.