Roche LightCycler 2.0 Thermal Cycler

• Real-time quantitative PCR (qPCR) for gene expression analysis.
• Pathogen detection and quantification in clinical and environmental samples.
• Genetic variation analysis, including SNP genotyping and mutation detection.
• DNA methylation analysis for epigenetic studies.
• Drug discovery and development for target validation and screening.

Typical reaction volume

Commonly supports reaction volumes in the range of 5-20 µL, utilising capillaries.

Temperature range

Typically operates across a broad temperature range suitable for PCR, commonly 35-95°C.

Heating/cooling rate

Known for rapid temperature ramping, often exceeding 10°C/s for heating and cooling.

Detection channels

Units of this type commonly feature multiple fluorescence detection channels, typically 3-6, allowing for multiplexing.

Sample format

This model typically uses proprietary glass capillaries rather than standard PCR plates.

Power supply

For this class of equipment, a standard laboratory power supply, typically 230V, 50Hz (UK mains), is required.

Footprint

Units are generally compact benchtop devices, suitable for most laboratory environments.

• What consumables are required for this thermal cycler?

  This unit typically requires specific Roche LightCycler capillaries and associated reagents. Buyers should confirm availability and compatibility with their current stock.

• Is specialist software needed to operate this equipment?

• What level of maintenance does this unit typically require?

• Can this unit perform high-resolution melt (HRM) analysis?

• What are the typical data output formats?

PCR machines and thermal cyclers are regarded as reliable workhorses in molecular biology laboratories. Buyers typically value precision temperature control, consistent cycling performance, and straightforward programming interfaces. When purchasing used equipment, inspectors should verify heating block accuracy, check thermal uniformity across wells, and confirm software compatibility with current laboratory systems. Common strengths include durability, ease of maintenance, and sustained performance across numerous applications in research, diagnostics, and quality control settings.

General overview for this equipment type