Spectramax M2 Microplate reader

• ELISA assays are commonly performed for detecting antibodies, antigens, proteins, and glycoproteins.
• DNA and RNA quantification is a typical application for determining nucleic acid concentrations.
• Protein quantification, such as Bradford or BCA assays, is frequently carried out.
• Enzyme kinetics studies are often conducted to measure reaction rates and enzyme activity.
• Cell-based assays, including cell viability and proliferation, are a common use for this equipment.

Plate Formats

Typically accommodates 6- to 384-well microplates.

Detection Modes

Commonly includes absorbance, fluorescence (top-read), and often luminescence and bottom-read fluorescence on enhanced models.

Wavelength Selection

Units of this type typically feature dual monochromators for selectable excitation and emission wavelengths.

Pathlength Correction

Many models incorporate automatic pathlength normalisation for absorbance readings.

Throughput

For this class of equipment, throughput is generally high, especially when integrated with automation systems.

Power Supply

Typically operates on standard laboratory power, often 100-240V AC, 50/60 Hz.

Footprint

These are generally compact benchtop instruments, suitable for standard laboratory benches.

• What types of assays can this Spectramax M2 reader perform?

  This unit is designed for a variety of life science applications, typically including ELISA, DNA/protein quantification, enzyme kinetics, and cell-based assays, utilising absorbance and fluorescence detection modes.

• Does this unit require specific software?

• Is this microplate reader suitable for high-throughput applications?

• What is the benefit of dual monochromators?

• Can this unit read samples in formats other than microplates?

Microplate readers remain essential laboratory instruments valued for their reliability and consistency in high-throughput screening applications. Buyers typically prioritise optical performance, wavelength range compatibility, and software functionality when evaluating used models. Key considerations include detector condition, calibration currency, and spare parts availability. The equipment generally performs well in pharmaceutical development, diagnostics, and research settings. Reputation centres on durability and cost-effectiveness, particularly when sourced from well-maintained laboratory environments with complete documentation.

General overview for this equipment type