Spectrophotometers (UV-Vis, Fluorescence, Colorimeters)
Spectrophotometers measure how a sample interacts with light to determine concentration, purity or colour, making them one of the most widely used instruments on any laboratory bench. This category covers UV-Vis and fluorescence spectrophotometers alongside colorimeters, from compact single-cell units to scanning double-beam systems. Buyers can compare wavelength range, bandwidth and cell options, then contact sellers directly about lamp condition and software. Financing on qualifying listings makes it easy to equip routine or research measurement.
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Spectrophotometer Types
Instruments are grouped by the optical measurement they make, which determines the assays and samples they handle. The variants below cover the common formats.
UV-Vis spectrophotometers
Instruments that measure absorbance across ultraviolet and visible wavelengths for concentration and purity work. Single and double-beam designs suit routine and higher-precision needs.
Fluorescence spectrophotometers
Systems that excite a sample and measure emitted light for sensitive, selective assays. They detect lower concentrations than absorbance for suitable fluorophores.
Colorimeters
Filter-based instruments that measure colour at set wavelengths for fast, economical routine assays. They are a practical choice for high-volume colorimetric testing.
Micro-volume and nucleic acid readers
Compact instruments that measure tiny sample volumes for DNA, RNA and protein quantification. They save precious sample compared with standard cuvette measurement.
Brands on Machinery Masters
Sellers list spectrophotometers from established laboratory instrument makers with dependable lamp and service support. The names below are drawn from live Spectrophotometers (UV-Vis, Fluorescence, Colorimeters) listings and refresh as sellers add and sell equipment.
Latest Spectrophotometers (UV-Vis, Fluorescence, Colorimeters) Listings
Why Choose Machinery Masters
Verified Sellers Only
Every seller is verified before a single listing goes live. Machinery Masters also reviews listings for accuracy, so buyers can commit to high value machinery with confidence.
Global Reach
Listings come from sellers across Europe, North America, and beyond. That reach widens the options past any regional dealer network, and it is often the only way to find discontinued models in good condition.
Flexible Financing Options
Qualifying listings carry financing through partner providers. Terms are shown directly on eligible product pages, spreading the cost instead of draining working capital in one hit.
Direct Seller Contact
No broker sits between the buyer and the seller. Ask about service history, arrange inspections, and negotiate directly with the person who actually knows the machine.
Applications and Uses
Spectrophotometry supports measurement across research, QC and teaching labs. The settings below show typical use.
Concentration and purity determination
Labs quantify compounds and check purity by measuring absorbance at defined wavelengths. This routine task underpins much of daily analytical work.
Nucleic acid and protein quantification
Analysts read absorbance ratios to quantify and assess the quality of DNA, RNA and protein. Micro-volume readers make this efficient with small samples.
Colorimetric assay screening
Colorimeters measure the colour change of enzymatic and chemical assays across many samples. Their speed suits high-volume routine testing.
Environmental and water analysis
Testing labs quantify nutrients and contaminants using absorbance-based methods. Reliable measurement supports compliance monitoring.
Benefits from Machinery Masters
Part of Analytical Instruments & Detection Systems, this category brings spectrophotometers together and puts you in touch with sellers who know each instrument's condition.
The full span of optical measurement
UV-Vis, fluorescence and colorimeter spectrophotometers appear together for straightforward comparison. That helps you match the measurement type to your assays without hunting across suppliers.
Dependable readings at a used-instrument cost
A well-kept used spectrophotometer performs the same routine measurements as a new model for far less. That leaves budget for cuvettes, lamps and support.
Lamp and cell compatibility from the seller
You confirm lamp hours, wavelength range and cell options directly with the owner. Those specifics decide whether a spectrophotometer suits your work.
Frequently Asked Questions About Spectrophotometers (UV-Vis, Fluorescence, Colorimeters)
Match the instrument's wavelength range and spectral bandwidth to your methods, as UV work needs coverage below the visible range. Narrower bandwidth gives better resolution for demanding measurements. Confirm these specifications with the seller against your requirement. Choosing the right optics ensures the instrument suits your assays.
Lamps are consumables with a limited life, so ask about lamp hours and whether a recent replacement was fitted. UV-Vis instruments typically use deuterium and tungsten lamps that age with use. Confirm whether spare lamps are available for the model. Lamp condition strongly affects measurement stability and running cost.
Confirm the sample compartment accepts the cuvette path lengths and formats you use, including any flow cells or micro-volume options. Some instruments include specialised holders for particular sample types. Ask whether any cell accessories come with the unit. Matching cell compatibility keeps the instrument ready for your samples.
Confirm whether the spectrophotometer runs standalone or needs PC software, and whether that software transfers. Ask which data output and storage options are available. Some instruments store methods and results onboard for convenience. Clear answers here help you plan how results will be captured.
You agree condition, price and delivery directly with the seller once the instrument suits your needs. On eligible listings, finance can spread a spectrophotometer's cost across a set term. Check the individual listing to see whether finance applies. Dealing with the owner keeps the process clear.