Cytiva (formerly part of GE Healthcare Life Sciences) - AxiChrom 140/500 Chromatography Column

• Pilot-scale purification of biomolecules in biopharmaceutical development.
• Chromatographic separation in academic and industrial research laboratories.
• Intermediate-scale production of proteins, peptides, and nucleic acids.
• Process development and optimisation for larger-scale manufacturing.
• Purification steps in vaccine production and diagnostics.

Column diameter

This model number indicates a column diameter of 140 mm.

Column length

This model number indicates a column length of 500 mm.

Typical bed volume

For this class of equipment, bed volumes are typically in the range of 5 to 10 litres, depending on the packed bed height.

Operating pressure

Chromatography columns of this type commonly operate at pressures up to 20 bar (290 psi), though specific limits depend on the resin and application.

Construction materials

Units are typically constructed from biocompatible materials such as stainless steel (316L) and borosilicate glass, with wetted parts often PEEK or PTFE.

Temperature range

Commonly designed for operation between 4°C and 40°C, suitable for biological samples.

Automation compatibility

AxiChrom columns are typically designed for integration with automated chromatography systems, facilitating reproducible packing and operation.

• What is the primary benefit of an AxiChrom column?

  AxiChrom columns are known for their automated packing capabilities, which aim to provide highly reproducible and efficient separation, particularly important for scalable purification processes.

• Is this column suitable for cGMP environments?

• What type of chromatography resins can be used with this column?

• What kind of maintenance is typically required for these columns?

• Does this unit come with a packing station or control system?

Chromatography columns and accessories hold strong reputations within analytical laboratories for reliability and consistent performance. Buyers typically value used equipment that maintains original specifications and comes with documentation of maintenance history. Industry sentiment favours purchasing from reputable sources where column condition, packing integrity, and compatibility with existing systems are verified. Common strengths include durability of quality columns across multiple applications and cost savings versus new purchases. When buying used, inspecting for damage, confirming proper storage conditions, and verifying suitability for intended analytical methods remain critical considerations for laboratory operations.

General overview for this equipment type