GE Healthcare Ettan IPGphor 3 Isoelectric Focusing System

• Protein separation based on isoelectric point in proteomics research.
• First dimension of 2-D electrophoresis for complex protein mixture analysis.
• Preparation of samples for downstream mass spectrometry or Western blotting.
• Analysis of protein isoforms and post-translational modifications.
• Quality control in biopharmaceutical development and production.

Typical capacity

Capable of running up to 12 IPG strips simultaneously, commonly accommodating strip lengths from 7 cm to 24 cm.

Power supply

Units of this type typically operate from a standard laboratory mains supply, commonly 230V, 50Hz for the UK market.

Temperature control

Often includes integrated or external temperature control for consistent focusing conditions.

Control interface

Typically managed via dedicated software, allowing for programming and monitoring of multiple units.

Safety features

Commonly includes safety interlocks, such as automatic voltage cutoff when the lid is opened.

Compatibility

Often designed to be compatible with earlier models from the same series and suitable for 2-D DIGE applications.

• What is the primary function of an Isoelectric Focusing System?

  Its primary function is to separate proteins based on their isoelectric point (pI) in a pH gradient. This is often the first step in 2-D electrophoresis.

• Can this system be used for different IPG strip lengths?

• Is specialised software required to operate this equipment?

• What are the common maintenance requirements for this type of system?

• Is this system suitable for high-throughput protein analysis?

Isoelectric focusing systems hold solid reputation in molecular biology and genomics research. Buyers typically value their precision in protein separation and consistent reproducibility across runs. When purchasing used equipment, key considerations include chamber condition, power supply functionality, and availability of replacement buffers and consumables. These systems are praised for reliable performance in established protocols, though maintenance records and calibration history prove important for assessing equipment longevity and future operational costs.

General overview for this equipment type