GE Healthcare Ettan IPGphor 3 Isoelectric Focusing System

• Protein separation based on isoelectric point in proteomics research.
• First-dimension separation in 2-D electrophoresis workflows.
• Analysis of protein isoforms and post-translational modifications.
• Preparation of samples for 2-D DIGE applications.
• Quality control and characterisation of protein samples in biopharmaceutical development.

Typical strip processing capacity

Units of this type commonly process up to 12 IPG strips simultaneously.

Compatible IPG strip lengths

This class of equipment typically accommodates strip lengths of 7, 11, 13, 18, or 24 cm.

Control system

Advanced control software is typically used, often allowing management of multiple units.

Safety features

Commonly includes automatic voltage cutoff mechanisms for operator safety.

Power supply

Typically operates on standard laboratory electrical supplies; specific voltage and current requirements should be confirmed with the seller.

Sample handling

Often features sample cups for increased protein loading and improved separation efficiency.

• What is isoelectric focusing (IEF)?

  IEF is a technique for separating proteins based on their isoelectric point (pI), the pH at which a protein carries no net electrical charge. Proteins migrate through a pH gradient until they reach their pI.

• What are IPG strips?

• Can this system be used for 2-D electrophoresis?

• Is this unit compatible with existing laboratory equipment?

• What maintenance is typically required for an IEF system?

Isoelectric focusing systems hold solid reputation in molecular biology and genomics research. Buyers typically value their precision in protein separation and consistent reproducibility across runs. When purchasing used equipment, key considerations include chamber condition, power supply functionality, and availability of replacement buffers and consumables. These systems are praised for reliable performance in established protocols, though maintenance records and calibration history prove important for assessing equipment longevity and future operational costs.

General overview for this equipment type