Isoelectric Focusing Systems
Isoelectric focusing separates proteins by their isoelectric point, driving each molecule through a pH gradient until its net charge is zero. Isoelectric focusing systems provide the units, immobilised gradient strips and supporting hardware that make that separation possible, including the first dimension step of two dimensional gel workflows. High and stable voltage delivery is central to sharp resolution, so the power characteristics matter as much as the tray design. Buyers running proteomics within a molecular biology and genomics facility will find these systems and accessories here from verified sellers, with configuration detailed upfront.
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Isoelectric Focusing System Types
The formats below vary in gradient design, sample capacity and how tightly they control temperature during a run.
IPG strip focusing trays
These systems resolve proteins on immobilised pH gradient strips as the first dimension of a two dimensional workflow. Strip length and the number of channels set the resolution and the sample number per run.
Tube and slab gel focusing units
Traditional units run the separation in tube or slab gels with carrier ampholytes to form the gradient. They remain useful for established protocols and preparative work.
High voltage focusing power supplies
This step needs stable high voltage delivery, often several thousand volts, held steady through the run. Output range and volt hour programming decide how sharply proteins resolve.
Temperature controlled focusing platforms
Some models integrate active cooling to hold the gel at a set temperature during the run. Controlled temperature keeps the gradient stable and prevents heat driven artefacts.
Latest Isoelectric Focusing Systems Listings
Why Choose Machinery Masters
Verified Sellers Only
Every seller is verified before a single listing goes live. Machinery Masters also reviews listings for accuracy, so buyers can commit to high value machinery with confidence.
Global Reach
Listings come from sellers across Europe, North America, and beyond. That reach widens the options past any regional dealer network, and it is often the only way to find discontinued models in good condition.
Flexible Financing Options
Qualifying listings carry financing through partner providers. Terms are shown directly on eligible product pages, spreading the cost instead of draining working capital in one hit.
Direct Seller Contact
No broker sits between the buyer and the seller. Ask about service history, arrange inspections, and negotiate directly with the person who actually knows the machine.
Applications and Uses
This method supports protein separation wherever charge based resolution is needed. These settings show its main uses.
Two dimensional proteomics
It forms the first dimension of 2D gel electrophoresis, separating proteins by charge before size. High resolution here is what makes the second dimension worthwhile.
Protein charge variant analysis
Biopharma labs resolve charge variants of therapeutic proteins and antibodies. The technique helps confirm consistency between production batches.
Enzyme and isoform characterisation
Researchers separate closely related isoforms that differ only in isoelectric point. This reveals differences that size based methods miss.
Purity assessment of purified proteins
A run checks a purified protein preparation for charge based impurities. It complements other assays in building a full purity picture.
Benefits from Machinery Masters
The marketplace keeps this hardware within the wider protein separation range.
Isoelectric focusing hardware in one grouping
Trays, power supplies and cooling accessories sit together so a full first dimension setup is easy to assemble. Matching the parts is straightforward when they share a catalogue.
Specialist units at a used price
These units are niche, so a serviced, used one offers real savings over buying new. Listings describe the format, voltage range and included trays so the value is clear.
Confirm run detail with the seller
You can ask about typical volt hour programmes and included strip holders before buying. That direct exchange fills the gaps a listing photo cannot.
Frequently Asked Questions About Isoelectric Focusing Systems
Confirm the high voltage supply reaches and holds its rated output, since stable voltage is what gives sharp bands. Check the tray electrodes and any cooling plate are intact and free of salt corrosion. Ask for a recent run record to see the unit still resolves as expected.
Immobilised pH gradient strips give reproducible, defined ranges and are the usual choice for 2D proteomics. Narrow range strips resolve close variants, while broad range strips give a whole proteome overview. Match the strip range to the isoelectric points you need to separate.
Heat generated at high voltage can distort the gradient and blur bands, so many platforms cool the gel actively. If your protocol runs at high volt hours, a temperature controlled model is worth the extra cost. For shorter, lower voltage runs, passive cooling may be enough.
Trays use format specific strip holders and electrode wicks that must match the unit. Check these are included or still sold before you buy. The seller can confirm what ships with it and which consumables remain in production.
Every listing gives you a direct route to the seller, so you can ask about run history and included accessories straight away. There is no middleman between you and the owner. That direct contact is the quickest way to confirm a specialist instrument fits your protocol.