GE Healthcare (now Cytiva) - GE Healthcare Cytiva Axicrom 50/300t chromatography column

Applications & Use Cases

• Used for purification of biomolecules such as proteins, peptides, and nucleic acids in pharmaceutical and biotechnology research.
• Applied in process development and pilot-scale manufacturing for biopharmaceuticals.
• Suitable for separation and purification steps in vaccine production.
• Utilised in the isolation of active pharmaceutical ingredients (APIs) from complex mixtures.
• Employed in academic and industrial laboratories for preparative chromatography.

Typical Specifications

Internal Diameter (ID)

Typically 50 mm for this specific model, indicating the column's cross-sectional area.

Bed Height

Commonly 300 mm for this configuration, defining the length of the packed chromatography medium.

Operating Pressure

Units of this type typically operate at pressures suitable for preparative chromatography, often up to 10-20 bar.

Materials of Construction

Wetted parts are commonly made from biocompatible and chemically resistant materials such as 316L stainless steel, PEEK, and specific elastomers, meeting regulatory standards like USP Class VI.

Compression Mechanism

Features axial compression, where the packing bed is hydraulically compressed to ensure uniform density and prevent void formation.

Temperature Range

Typically designed to operate within a standard laboratory temperature range, often 4°C to 40°C.

Frequently Asked Questions

• What is the primary advantage of axial compression in this column?

  Axial compression helps to maintain a uniformly packed bed, which is crucial for high-resolution separations and consistent performance over multiple runs. It also simplifies repacking and reduces downtime.

• Can this column be used with different chromatography media?

• What kind of maintenance does an Axicrom column typically require?

• Is this column suitable for GMP environments?

• What is the typical flow rate range for a column of this size?