What is Tryptone Soya Broth (TSB) used for?

TSB is a general-purpose nutrient medium widely used for cultivating a broad spectrum of microorganisms, including bacteria and fungi. It is particularly valuable for sterility testing, microbial limit testing, and general microbial growth in various industries.

Does this product require refrigeration?

Dehydrated Tryptone Soya Broth typically does not require refrigeration prior to reconstitution. It is usually stored in a cool, dry place, protected from light and moisture, at room temperature.

How is TSB prepared for use?

TSB powder is typically dissolved in purified water according to the manufacturer's instructions, then sterilised, commonly by autoclaving. The specific concentration and sterilisation parameters will be detailed on the product label.

What is the significance of EP/USP/BP/JP compliance?

EP/USP/BP/JP compliance indicates that the product meets the stringent quality and performance standards set by the European, United States, British, and Japanese Pharmacopoeias, respectively. This is crucial for pharmaceutical and medical device applications where regulatory adherence is paramount.

Can this medium be used for anaerobic bacteria?

While TSB is a general-purpose medium, it is primarily aerobic. For culturing strict anaerobes, specific anaerobic conditions or a more specialised medium might be required, though TSB can support facultative anaerobes.

used Equipment

Available

Verified seller

Oxoid - Tryptone Soya Broth 10Kg

Name: Tryptone Soya Broth 10Kg
Brand: Oxoid
SKU: TSB0910
Price: 245 GBP
Availability: InStock

Price: £ 245

10Kg Thermo Scientific™ Tryptone Soy Broth (Soybean Case in Digest Medium EP/USP/BP/JP) (Dehydrated)

New in original packaging

Dated for use by end January 2026

Representing a price reduction of around 80% from the manufacturers retail price list

Soybean Casein Digest (SCD), also known as Soybean-Casein Digest Medium or Tryptic Soy Broth (TSB), is a general-purpose nutrient medium used for cultivating a wide range of microorganisms, including bacteria and fungi. It's especially valuable because it's rich in nutrients derived from enzymatic digests of soybean meal and casein (milk protein)

Used By

1. Pharmaceutical Industry

Sterility testing of pharmaceutical products (especially injectables and ophthalmic solutions).
Microbial limit testing of raw materials, finished products, and water.
Environmental monitoring (e.g., surface swabs, air sampling).

2. Medical Device Manufacturers

Used for sterility testing of surgical instruments, implants, catheters, etc.

3. Hospitals & Clinical Laboratories

For culturing bacteria and fungi from clinical specimens.
Used in blood culture bottles to detect septicemia.

4. Food and Beverage Industry

To test for microbial contamination in food products.
Used for hygiene monitoring in food processing environments.

5. Biotechnology and Research Laboratories

General purpose medium for growing a variety of microbes in research.
Useful in antibiotic sensitivity testing or microbiome studies.

6. Cosmetic Industry

For microbial limit testing and product safety validation.

Cambridgeshire, United Kingdom

5,791 km away

Model Number

Tryptone

Stock Number

TSB0910

Machinery Masters View

Typical context for this category of equipment. Always confirm specifics with the seller.

Applications & Use Cases

Sterility testing and microbial limit testing in the pharmaceutical and medical device industries.
Culturing bacteria and fungi from clinical specimens in hospitals and clinical laboratories.
Testing for microbial contamination and hygiene monitoring in the food and beverage industry.
General purpose microbial growth in biotechnology and research laboratories.
Microbial limit testing in the cosmetic industry.

Typical Specifications

Typical capacity: This listing is for a 10kg unit; Tryptone Soya Broth is commonly available in various bulk sizes, typically from 500g to 25kg.
Form: This product is dehydrated, requiring reconstitution with purified water prior to use.
Storage requirements: Typically stored in a cool, dry place, away from direct light, often at room temperature (15-25°C).
Shelf life: Dehydrated media commonly have a shelf life of several years when stored correctly, as indicated by the 'use by' date.
Regulatory compliance: For this class of product, compliance with pharmacopoeial standards (EP/USP/BP/JP) is common for pharmaceutical applications.
Preparation: Requires precise weighing and dissolution in purified water, followed by sterilisation (e.g., autoclaving) before use.

Frequently Asked Questions

TSB is a general-purpose nutrient medium widely used for cultivating a broad spectrum of microorganisms, including bacteria and fungi. It is particularly valuable for sterility testing, microbial limit testing, and general microbial growth in various industries.

Machinery Masters Intelligence. General guidance for this category of equipment only, not a substitute for the seller's exact specifications. Always confirm details with the seller.