Does TSB require sterilisation before use?

Yes, after reconstitution from its dehydrated form, TSB typically requires sterilisation, commonly by autoclaving, to ensure it is aseptic before inoculation with microbial samples.

What are the key ingredients in TSB?

TSB is rich in nutrients derived from enzymatic digests of soybean meal (tryptone) and casein (milk protein), providing amino acids, peptides, and other growth factors essential for microbial proliferation.

Can TSB be used for anaerobic cultures?

While TSB is primarily an aerobic medium, it can support the growth of some facultative anaerobes. For strict anaerobes, specific anaerobic media and conditions are typically required.

How should prepared TSB be stored?

Prepared (reconstituted and sterilised) TSB should typically be stored at 2-8°C and used within a specified period, often a few weeks, to maintain its efficacy and prevent contamination.

Used Tryptone Soya Broth 5Kg - Lab Organisation & Storage, photo 1

Used Tryptone Soya Broth 5Kg - Lab Organisation & Storage

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Oxoid - Tryptone Soya Broth 5Kg

Name: Tryptone Soya Broth 5Kg
Brand: Oxoid
SKU: TSB0910a
Price: 90 GBP
Availability: InStock

Price: £ 90

5Kg Thermo Scientific™ Tryptone Soy Broth (Soybean Case in Digest Medium EP/USP/BP/JP) (Dehydrated)

New in original packaging

Dated for use by end January 2026

Representing a price reduction of around 80% from the manufacturers retail price list

Soybean Casein Digest (SCD), also known as Soybean-Casein Digest Medium or Tryptic Soy Broth (TSB), is a general-purpose nutrient medium used for cultivating a wide range of microorganisms, including bacteria and fungi. It's especially valuable because it's rich in nutrients derived from enzymatic digests of soybean meal and casein (milk protein)

Used By

1. Pharmaceutical Industry

Sterility testing of pharmaceutical products (especially injectables and ophthalmic solutions).
Microbial limit testing of raw materials, finished products, and water.
Environmental monitoring (e.g., surface swabs, air sampling).

2. Medical Device Manufacturers

Used for sterility testing of surgical instruments, implants, catheters, etc.

3. Hospitals & Clinical Laboratories

For culturing bacteria and fungi from clinical specimens.
Used in blood culture bottles to detect septicemia.

4. Food and Beverage Industry

To test for microbial contamination in food products.
Used for hygiene monitoring in food processing environments.

5. Biotechnology and Research Laboratories

General purpose medium for growing a variety of microbes in research.
Useful in antibiotic sensitivity testing or microbiome studies.

6. Cosmetic Industry

For microbial limit testing and product safety validation.

Cambridgeshire, United Kingdom

5,791 km away

Model Number

Tryptone

Stock Number

TSB0910a

Machinery Masters View

Typical context for this category of equipment. Always confirm specifics with the seller.

Applications & Use Cases

Sterility testing and microbial limit testing in the pharmaceutical industry.
Sterility testing of medical devices such as surgical instruments and implants.
Culturing bacteria and fungi from clinical specimens in hospitals and clinical laboratories.
Testing for microbial contamination and hygiene monitoring in the food and beverage industry.
General purpose microbial growth in biotechnology and research laboratories.

Typical Specifications

Typical format: Dehydrated powder, requiring reconstitution with purified water.
Common pack sizes: Typically available in 500g, 2.5kg, and 5kg containers for laboratory and industrial use.
Storage conditions: Commonly requires storage in a cool, dry place, away from direct light, for optimal shelf life.
Typical pH range (reconstituted): For this class of medium, the pH is typically around 7.3 ± 0.2 at 25°C after reconstitution.
Certifications: Units of this type are commonly manufactured to EP/USP/BP/JP standards for pharmaceutical applications.
Shelf life: Typically several years from the date of manufacture when stored correctly, with specific expiry dates provided by the manufacturer.

Frequently Asked Questions

TSB is a general-purpose nutrient medium widely used for the cultivation of a broad range of microorganisms, including bacteria and fungi, across various industries such as pharmaceuticals, food, and clinical diagnostics.

Machinery Masters Intelligence. General guidance for this category of equipment only, not a substitute for the seller's exact specifications. Always confirm details with the seller.