What is the primary function of an Akta Crossflow system?

The primary function is tangential flow filtration (TFF), used for concentrating, diafiltering (buffer exchange), and clarifying biological samples such as proteins, viruses, and plasmids.

Can this system be used for both ultrafiltration and microfiltration?

Yes, Akta Crossflow systems are typically versatile and can accommodate both ultrafiltration and microfiltration membranes, depending on the specific cassette installed.

Is this unit suitable for GMP environments?

Many Akta Crossflow systems are designed with features like CIP capabilities and robust control, making them suitable for use in regulated GMP environments. Buyers should verify specific unit documentation.

What types of samples can be processed?

This equipment is commonly used for processing a wide range of biological samples, including proteins, antibodies, viral vectors, plasmid DNA, and cell cultures.

Are consumables, such as filtration cassettes, readily available?

Filtration cassettes and other consumables for Akta Crossflow systems are widely available from Cytiva and third-party suppliers, ensuring ongoing operational support.

Used Akta Crossflow Filtration System - Pharmaceutical Separators, photo 1

Used Akta Crossflow Filtration System - Pharmaceutical Separators, photo 2

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Used Akta Crossflow Filtration System - Pharmaceutical Separators, photo 4

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Used Akta Crossflow Filtration System - Pharmaceutical Separators, photo 12

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Used Akta Crossflow Filtration System - Pharmaceutical Separators

used Equipment

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Verified seller

Akta PD - Akta Crossflow Filtration System

Name: Akta Crossflow Filtration System
Brand: Akta PD
SKU: 41683.1A
Availability: InStock

Price: Contact seller for price

This listing is for the Akta crossflow filtration system

The unit is in full working condition and is ready for immediate use

An ÄKTA Crossflow filtration system is a type of tangential flow filtration (TFF) system developed by Cytiva (formerly GE Healthcare). It's used primarily in bioprocessing and biotechnology applications to concentrate, diafilter, or clarify biological samples such as proteins, viruses, plasmids, and other biomolecule

Core Functions:

Concentration – Reducing the volume of a sample while retaining the target biomolecule.

Diafiltration (Buffer Exchange) – Removing salts or changing the buffer environment by continuously adding a new buffer while removing permeate.

Clarification – Removing particulates or cells from process fluids

Key Components:

Pump Modules: Typically peristaltic pumps that manage flow rates precisely.

Filtration Cassettes: Hollow fiber or flat sheet membranes for ultrafiltration or microfiltration.

Pressure Sensors: To monitor and control transmembrane pressure (TMP).

Conductivity/pH/UV Detectors: For in-line monitoring of the filtrate and retentate.

Benefits:

Automation reduces operator error and variability.

Scalable design for seamless transition from lab to production.

Precise control of filtration parameters (flow rate, pressure, volume).

CIP (Clean-In-Place) capabilities for easy maintenance and compliance with GMP.

Common Applications:

Protein concentration and buffer exchange

Viral vector purification

Plasmid DNA purification

Cell harvest and clarification

Cambridgeshire, United Kingdom

5,791 km away

Model Number

Crossflow

Stock Number

41683.1A

Machinery Masters View

Typical context for this category of equipment. Always confirm specifics with the seller.

Applications & Use Cases

Concentration and buffer exchange of proteins and other biomolecules.
Purification of viral vectors and plasmid DNA.
Clarification of cell cultures and fermentation broths.
Removal of salts or changing buffer environments for sensitive samples.
Preparation of samples for chromatography or further downstream processing.

Typical Specifications

Typical throughput: For this class of equipment, throughput can range from millilitres to several litres per hour, depending on membrane area and application.
Power supply: Units commonly operate on standard laboratory power, typically 230V, 50Hz in the UK.
Membrane types: Typically compatible with ultrafiltration or microfiltration membranes, often in hollow fibre or flat sheet cassette formats.
Operating pressure: Commonly features precise pressure control for transmembrane pressure (TMP) monitoring, critical for process efficiency.
Automation level: Units of this type typically offer high levels of automation for flow rate, pressure, and volume control.
Materials of construction: Wetted parts are commonly made from biocompatible materials such as stainless steel and pharmaceutical-grade polymers.
Maintenance: Features like Clean-In-Place (CIP) capabilities are commonly found, simplifying cleaning and sterilisation.

Frequently Asked Questions

The primary function is tangential flow filtration (TFF), used for concentrating, diafiltering (buffer exchange), and clarifying biological samples such as proteins, viruses, and plasmids.

Machinery Masters Intelligence. General guidance for this category of equipment only, not a substitute for the seller's exact specifications. Always confirm details with the seller.