Akta PD - Akta Crossflow Filtration System

Applications & Use Cases

• Concentration and buffer exchange of proteins and other biomolecules.
• Purification of viral vectors and plasmid DNA.
• Clarification of cell cultures and fermentation broths.
• Removal of salts or changing buffer environments for sensitive samples.
• Preparation of samples for chromatography or further downstream processing.

Typical Specifications

Typical throughput

For this class of equipment, throughput can range from millilitres to several litres per hour, depending on membrane area and application.

Power supply

Units commonly operate on standard laboratory power, typically 230V, 50Hz in the UK.

Membrane types

Typically compatible with ultrafiltration or microfiltration membranes, often in hollow fibre or flat sheet cassette formats.

Operating pressure

Commonly features precise pressure control for transmembrane pressure (TMP) monitoring, critical for process efficiency.

Automation level

Units of this type typically offer high levels of automation for flow rate, pressure, and volume control.

Materials of construction

Wetted parts are commonly made from biocompatible materials such as stainless steel and pharmaceutical-grade polymers.

Maintenance

Features like Clean-In-Place (CIP) capabilities are commonly found, simplifying cleaning and sterilisation.

Frequently Asked Questions

• What is the primary function of an Akta Crossflow system?

  The primary function is tangential flow filtration (TFF), used for concentrating, diafiltering (buffer exchange), and clarifying biological samples such as proteins, viruses, and plasmids.

• Can this system be used for both ultrafiltration and microfiltration?

• Is this unit suitable for GMP environments?

• What types of samples can be processed?

• Are consumables, such as filtration cassettes, readily available?