Spectrum Labs - Spectrum Labs KrosFLO TFF System KMPi Auxiliary Pump for Diafiltration

Applications & Use Cases

• Concentration and diafiltration of proteins, viruses, and nanoparticles in biopharmaceutical research.
• Clarification of cell cultures and fermentation broths in laboratory settings.
• Small-scale purification of vaccines and therapeutic proteins.
• Buffer exchange and desalting of biological samples.
• R&D-scale microfiltration and ultrafiltration processes for process development.

Typical Specifications

Typical processing volume

For this class of equipment, processing volumes commonly range from a few millilitres up to 10-15 litres.

Pump type

Units of this type typically feature peristaltic pumps, suitable for handling various fluid types with minimal shear.

Filtration mode

Designed for tangential flow filtration (TFF), utilising either hollow fibre filters or flat sheet cassettes.

Power supply

Commonly operates on standard laboratory power, typically 230V, 50Hz for the UK market.

Control interface

Modern systems often include a tablet or touchscreen interface for ease of operation and real-time monitoring.

Footprint

Typically designed with a compact footprint, suitable for benchtop use in laboratory environments.

Scale integration

Many systems integrate a scale for gravimetric control and accurate volume monitoring during processing.

Frequently Asked Questions

• What is the primary function of an auxiliary pump in a TFF system?

  An auxiliary pump, such as the KMPi, is typically used to manage the diafiltration buffer flow, enabling efficient buffer exchange and purification during the TFF process.

• Can this system handle different types of filters?

• Is this unit suitable for GMP environments?

• What kind of maintenance does a TFF system typically require?

• What are the benefits of tangential flow filtration over dead-end filtration?