Cytiva DE52 10 kg Whatman Ion Exchange Diethylaminoethyl Cellulose

• Protein purification in laboratory and industrial settings.
• Separation of biomolecules such as enzymes, antibodies, and nucleic acids.
• Ion exchange chromatography for research and production of pharmaceuticals.
• Desalting and buffer exchange of protein solutions.
• Fractionation of complex biological mixtures.

Typical capacity

For this class of diethylaminoethyl cellulose, binding capacities are typically high, often in the range of 0.8-1.2 meq/g, depending on the specific protein and buffer conditions.

Format

Commonly supplied as a dry powder or pre-swollen slurry, intended for packing into chromatography columns.

Particle size

Typically, DEAE cellulose resins feature particle sizes ranging from 40-100 µm, influencing flow rates and resolution.

pH stability

Units of this type are generally stable across a broad pH range, typically from pH 2 to pH 10, for operational use.

Chemical compatibility

Commonly compatible with standard buffers, salts, and denaturing agents used in protein purification.

Storage conditions

Typically stored dry at room temperature, away from direct light, to maintain integrity and shelf life.

• What is DEAE cellulose used for?

  DEAE cellulose is an anion exchange resin primarily used for the purification and separation of proteins, enzymes, and other biomolecules based on their charge properties.

• How should this material be prepared before use?

• Can this resin be regenerated and reused?

• What is the typical shelf life of unopened DEAE cellulose?

• Is this material suitable for large-scale industrial applications?

Chromatography columns and accessories hold strong reputations within analytical laboratories for reliability and consistent performance. Buyers typically value used equipment that maintains original specifications and comes with documentation of maintenance history. Industry sentiment favours purchasing from reputable sources where column condition, packing integrity, and compatibility with existing systems are verified. Common strengths include durability of quality columns across multiple applications and cost savings versus new purchases. When buying used, inspecting for damage, confirming proper storage conditions, and verifying suitability for intended analytical methods remain critical considerations for laboratory operations.

General overview for this equipment type