Cytiva DE52 10 kg Whatman Ion Exchange Diethylaminoethyl Cellulose

• Protein purification in laboratory and industrial settings.
• Separation of biomolecules such as enzymes, antibodies, and nucleic acids.
• Fractionation of complex biological mixtures.
• Polishing steps in biopharmaceutical manufacturing processes.
• Research and development in biochemistry and molecular biology.

Typical capacity

This specific listing is for a 10 kg unit. For this class of ion exchange media, binding capacities are typically expressed in meq/g or mg/mL, varying significantly based on the target molecule and operating conditions. Buyers should consult the manufacturer's data sheet for specific binding capacities relevant to their application.

Power supply

Ion exchange media itself does not require a power supply. It is used within chromatography systems which typically require standard laboratory power, commonly 230V, 50Hz in the UK.

Material type

Diethylaminoethyl (DEAE) cellulose, a weak anion exchanger. This material is commonly used for its robust chemical stability and good flow properties.

Operating pH range

For DEAE cellulose, the effective operating pH range is typically between pH 3 and pH 9, though optimal performance for specific separations may be narrower. Buyers should verify compatibility with their buffer systems.

Regeneration and cleaning

Units of this type are typically regenerated using salt solutions (e.g., NaCl) and cleaned with mild detergents or alkaline solutions. Specific protocols depend on contaminants and manufacturer guidelines.

Storage conditions

Unopened units are typically stored at ambient temperature in a dry place, away from direct sunlight. Once opened, specific recommendations may apply to maintain performance.

• What is the typical shelf life of unopened DEAE cellulose?

  Unopened, sealed units of DEAE cellulose typically have a shelf life of several years when stored according to manufacturer guidelines. Buyers should confirm the specific expiry date with the seller if critical.

• Can this DEAE cellulose be reused?

• Is this media compatible with organic solvents?

• What is the particle size of this DEAE cellulose?

• How is this media packed for shipping?

Chromatography columns and accessories hold strong reputations within analytical laboratories for reliability and consistent performance. Buyers typically value used equipment that maintains original specifications and comes with documentation of maintenance history. Industry sentiment favours purchasing from reputable sources where column condition, packing integrity, and compatibility with existing systems are verified. Common strengths include durability of quality columns across multiple applications and cost savings versus new purchases. When buying used, inspecting for damage, confirming proper storage conditions, and verifying suitability for intended analytical methods remain critical considerations for laboratory operations.

General overview for this equipment type